What is Qiagen protease?

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

What is the concentration of Qiagen Proteinase K?

20 mg/mL
What is the concentration of the different Proteinase K solutions sold by QIAGEN? Puregene Proteinase K and QIAGEN Proteinase K are both 20 mg/mL.

What is the difference between protease and Proteinase K?

The key difference between proteinase K and protease is that proteinase K is useful in digesting proteins and removing contamination from nucleic acid preparations, whereas protease is useful in biological functions such as digestion of ingested proteins, protein catabolism, and cell signaling.

What is Proteinase K made of?

Proteinase K is a typical member of the subtilisin family of proteinases (S8). The amino acid sequence has been derived by Edman degradation [2] and from the gene sequence [3]; the polypeptide chain consists of 278 amino acids, with molecular mass 28 930, and the pI is 8.9 [1].

Is proteinase K toxic?

Inhalation May cause allergic respiratory reaction. If breathing has stopped, give artificial respiration. Get medical attention immediately. Remove to fresh air.

How do you use proteinase K?

Proteinase K is used mostly in DNA and RNA extraction protocols. You’ll often find the proteinase K step within the lysis section of the protocol. For example, in the nucleic acid extraction protocol, proteinase K is added to cell lysate and then an incubation period follows to ensure a complete digestion.

How long are Qiagen kits good for?

QIAGEN Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the shelf-life of Proteinase K, storage at 2–8°C is recommended.

What is proteinase K used for?

Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.

What is proteinase used for?

Proteolytic enzymes (proteases) are enzymes that break down protein. These enzymes are made by animals, plants, fungi, and bacteria. Proteolytic enzymes break down proteins in the body or on the skin. This might help with digestion or with the breakdown of proteins involved in swelling and pain.

What does the K stand for in proteinase K?

The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album). Proteinase K is able to digest hair (keratin), hence, the name “Proteinase K”.

Why is grinding buffer toxic?

Harmful if swallowed. Material may be irritating to the mucous membranes and upper respiratory tract. May be harmful by inhalation or skin absorption. May cause skin or respiratory system irritation.

What is the importance of proteinase K?

Proteinase K is used during DNA extraction to digest many contaminating proteins present. It also degrades nucleases that may be present in DNA extraction and protects the nucleic acids from nuclease attack.

Does proteinase K expire?

How do you inactivate proteinase K?

Heat is a widely used way of inactivating proteinase K. While the activity of proteinase K increases with temperature, and is optimized at about 65 ˚C, heating proteinase K to 95 ˚C for 10 minutes will inactivate it. Keep in mind, however, that heating proteinase K does not fully inactivate the enzyme.

What is proteinase K used for in RNA extraction?

Proteinase K is critical because it digests proteins eliminating contamination from nucleic acid preparations, in addition to inactivating the nucleases that could degrade DNA or RNA during purification.

What are the products of protease?

Where enzymes are produced

Enzyme	Substrate	End-products
Protease	Protein	Amino acids
Lipase	Lipids (fats and oils)	Fatty acids and glycerol
Pancreatic amylase	Starch	Maltose
Maltase	Maltose	Glucose

Why is NaCl used in protein extraction?

Despite mimicking the physiological condition, NaCl can basically help to increase the ionic strength of the native or denaturing purification buffers. It helps to remove the contaminants from the Ni-NTA resin by reducing the non-specific hydrophobic and ionic interaction binding between the protein.

What is QIAGEN proteinase K?

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times.

What are the side effects of Proteinase K?

Proteinase KRevision Date 28-Dec-2021 Causes serious eye irritation May cause allergy or asthma symptoms or breathing difficulties if inhaled May cause respiratory irritation Precautionary Statements Prevention Wash face, hands and any exposed skin thoroughly after handling Wear protective gloves/protective clothing/eye protection/face protection

What is the pH of QIAGEN protease?

* pH 8.0, 50°C, 1.25 µg/ml protease, 15 min incubation. † Buffer G2 used in QIAGEN Genomic-tip procedures for DNA isolation from blood, cell cultures, and tissue.

Is there a TSCA inventory notification for proteinase K (TSCA)?

Component CAS No TSCA TSCA Inventory notification – Active-Inactive TSCA – EPA Regulatory Flags Page 5 / 7 Proteinase KRevision Date 28-Dec-2021 Proteinase K 39450-01-6 – – – Legend: TSCA US EPA (TSCA) – Toxic Substances Control Act, (40 CFR Part 710)