How do you dispose of BrdU?

Remaining BrdU solution, contaminated bench paper, gloves, etc should be disposed of through the OH&S Support Facility by incineration. Chronic administration -‐ BrdU may be administered to animals on a chronic basis by IV or IP injection, by osmotic pump or in their drinking water. 1.

What does BrdU staining do?

BrdU (Bromodeoxyuridine / 5-bromo-2′-deoxyuridine) is an analog of the nucleoside thymidine used in the BrdU assay to identify proliferating cells. BrdU labeling can be performed in vitro for cell lines and primary cell cultures, or in vivo for labeling cells within a living animal.

How is BrdU Incorporated?

During S phase of the cell cycle (when DNA replication occurs), BrdU can be incorporated in place of thymidine in newly synthesized DNA molecules of dividing cells.

Is BrdU a biohazard?

Disposal of BrdU All potentially contaminated animal carcasses, tissue samples, bedding, other laboratory or animal house materials and unused BrdU should be double bagged in biohazard bags, clearly labelled “Bromodeoxyuridine” and stored securely whilst awaiting disposal.

Is BrdU hazardous?

BrdU is considered hazardous as it has mutagenic and strong teratogenic properties. This agent disrupts the normal activity of the cell. It is a suspected carcinogen and therefore exposure should be kept as low as reasonably achievable.

How do you use BrdU?

Detect incorporated BrdU

1. Remove this solution and add 1 mL of antibody staining buffer.
2. Add anti-BrdU primary antibody.
3. Incubate overnight at room temperature.
4. Wash with Triton X-100 permeabilization buffer (3 times, 2 minutes each)
5. Add fluorescently labeled secondary antibody.
6. Incubate one hour at room temperature.

What does BrdU measure?

Bromodeoxyuridine (BrdU) incorporation assays have long been used to detect DNA synthesis in vivo and in vitro. The key principle of this method is that BrdU incorporated as a thymidine analog into nuclear DNA represents a label that can be tracked using antibody probes.

Is BrdU toxic?

Yes, BrdU (Bromodeoxyuridine) is toxic. It is also teratogenic, cytotoxic, and mutagenic. BrdU results in several adverse effects on cells that incorporate it. It increases the length of the cell cycle, alters DNA stability, initiates the formation of teratomas, and triggers cell death.

What does EdU stain for?

Product overview. EdU Assay / EdU Staining Proliferation Kit (iFluor 488) ab219801 provides a sensitive and robust method to detect and quantify cell proliferation in live mammalian cells using flow cytometry or fluorescence microscopy.

Is BrdU carcinogenic?

How do you store BrdU?

The molecular weight of BrdU is 307.1. Store product at -80°C prior to use or for long term storage of stock solutions. This preparation contains no preservatives, thus it should be handled under aseptic conditions. Avoid multiple freeze-thaws of product.

Is BrdU light sensitive?

BrdU is light sensitive and should be added in the dark. Cells pulsed with BrdU may be photosensitive — incubations should be in the dark as well.

What is the difference between EdU and BrdU?

EdU assay. Unlike assays using BrdU staining, Click-iT EdU assays are not antibody-based and therefore do not require DNA denaturation for detection of the incorporated nucleoside.

How stable is BrdU?

The BrdU solution once thawed is stable at 4°C for 4 months.

What is the concentration of BrdU solution?

The BrdU stock solution we keep is at a concentration of 10 mg/mL (in PBS I believe). We keep those stocks in the -20 deg C freezer. After thawing, some BrdU might come out of solution, so at that point we either vortex the tube for a little bit and/or warm up the tube so the powder goes into solution.

How do you dissolve BrdU powder?

We usually used NaOH (0.02N) to dissolve BrdU powder. For a final concentration of 100 mg BrdU / kg of animal we dissolve 33mg of BrdU in 1 ml of NaOH (Stock solution). This allow us to have more solubility.To prepare the injection solution, mix 1 part stock + 3 volumes of 0.9% NaCl in order to buffer the alkalinity (NaOH) of the stock solution.

What is the recommended usage of BrdU?

Recommended usage includes in vivo and in vitro measurement of DNA synthesis, study of the cell cycle, and labeling of proliferating cells. a) Administration in drinking water: Dilute the BrdU in drinking water to a concentration of 0.8-1.0 mg/ml. Give to animals for consumption ad libitum for up to 4 weeks.

What are the storage conditions for BrdU control slides ab129956?

Unstained control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956 . Store at -20°C. Store under desiccating conditions.