What is the PC3 cell line?

PC3 (PC-3) is a human prostate cancer cell line used in prostate cancer research and drug development. PC3 cells are useful in investigating biochemical changes in advanced prostate cancer cells and in assessing their response to chemotherapeutic agents.

What are du145 cells?

DU 145 is a cell line with epithelial morphology that was isolated from the brain of a 69-year-old, White, male with prostate cancer. This cell line is suitable as a transfection host. Homo sapiens, human. Morphology epithelial Tissue Prostate Disease. Carcinoma.

What does PC3 stand for?

PC3

Acronym	Definition
PC3	Prostate Cancer Cell Line (oncology)
PC3	Prohormone Convertase 3
PC3	Paul Coleman Trio (band)
PC3	Postal Clerk Third Class (Naval Rating)

Do PC3 cells express AR?

PC3 cells do not express AR and PSA and their proliferation is independent of androgen, similar to SCNC. Adenocarcinoma cells and LNCaP cells are negative for neuroendocrine markers and stem cell-associated marker CD44 while SCNC and PC3 cells are positive.

Is PC3 AR positive?

The PC-3 cell line that was derived from a human bone marrow prostate metastatic tumor35, is AR negative but expresses both CK5 and CK8, and therefore could be regarded as basal intermediate PCa cells.

What is PC-3 cell line used for?

The cell line is identical to the source provided by the depositor based on the STR-PCR analysis. PC-3 cell line has been used to study the antiproliferative effects of F. mughlea Pesmen on cancer cells. It has also been used to study the effects of palmitoylcarnitine (palcar) on the gene expression and pro-inflammatory pathways in prostate cancer.

How many PC3 cells are in a tissue culture dish?

For low density plating, ~8,000 PC3 cells were seeded in a 100 mm tissue culture dish to maintain the cell density between 50 and 200 cells per cm 2 [ 27 ]. For plating by limited dilution, each well of a 96-well plate was seeded with 100 μl culture medium containing a calculated 10 PC3 cells/ml [ 32 ].

Which markers do PC3 cells express but LNCaP cells do not?

PC3 cells express NE cell markers but LNCaP cells do not. A: Western blot assay shows that PC3 cells, not LNCaP cells, express NE markers chromogranin A and NSE. B,C: show the results of qPCR studies showing higher mRNA levels for CgA and NSE in PC3 cells than in LNCaP cells.

Are PC3 tumors supported by stem cells with stem-like characteristics?

These findings support the proposal that PC3 tumors are sustained by a small number of tumor-initiating cells with stem-like characteristics, including strong self-renewal and pro-angiogenic capability and marked by the expression pattern FAM65Bhigh/MFI2low/LEF1low .